7 - Biotecnología y Bioprocesos

ETHANOLIC EXTRACT OF THE ANTARCTIC ISOLATE Sphingomonas sp. UV9 AS A POTENTIAL PRO-HEALING AGENT

Pazos-Espinosa, Hany ¹ - Vila, María Eugenia ² - Davyt, Danilo ³ - Marizcurrena, Juan José ⁴ - Castro-Sowinski, Susana ⁴ - Alem, Diego ¹ - Canclini, Lucia ¹

1) Departamento de Genética del Instituto de Investigaciones Biológicas Clemente Estable- Montevideo - Montevideo - Uruguay
2) Departamento de Bioingeniería del Instituto de Ingeniería Química de Facultad de Ingeniería, Universidad de la República-Montevideo - Montevideo - Uruguay
3) Departamento de Química Orgánica de Facultad de Química, Universidad de la República-Montevideo - Montevideo - Uruguay
4) Sección Bioquímica y Biología Molecular de Facultad de Ciencias, Universidad de la República-Montevideo - Montevideo - Uruguay
Contacto: hany.pazos.espinosa@gmail.com

The process of skin healing is crucial for physical, aesthetic, and psychological well-being. Although there are several treatments to promote healing, their effectiveness is often limited, highlighting the ongoing need for new solutions. Currently, some Antarctic biomolecules with cosmetic applications for skin care are reported. However, there is little exploration of the use of Antarctic bacteria in promoting wound closure. In this study, we evaluated the potential of an ethanolic extract from Antarctic bacteria Sphingomonas sp. UV9 using both in vitro and in vivo healing models. In vitro assays in the HaCaT model revealed that the ethanolic extract of Sphingomonas sp. UV9 (ExUV9) exhibited dose-dependent pro-healing activity and stimulated cell proliferation and migration processes. Furthermore, it was proven that exposure to ExUV9 at the doses evaluated does not reduce cell viability, does not increase the frequency of micronucleus generation, and significantly reduces ɣH2Ax labeling compared to basal levels. Additionally, the ABTS assay showed that ExUV9 has antioxidant capacity. The properties of ExUV9 on the healing and skin regeneration process were also assessed using a murine excisional wound model. This in vivo assay suggested an enhanced wound healing process after 8 days of treatment with ExUV9. Studies on the growth kinetics of the microorganism revealed that the bioactive component of ExUV9 is produced from early stages, with kinetics similar to the production of primary metabolites in bacteria. ExUV9 promotes wound closure without inducing cytotoxicity or genotoxicity in the HaCaT model, making it a promising candidate for use as a therapeutic healing agent.

Palabras clave: Antartic bacteria - Sphingomonas sp. - wound healing - HaCaT - murine excisional wound model